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R&D Systems collagen i
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R&D Systems pro collagen i alpha 1
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Novus Biologicals antibodies against collagen i
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R&D Systems cultrex rat collagen i
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R&D Systems human procollagen iα1 duoset elisa kit
In vitro evaluation of the effects of Medipep-6PN on MMP-1 activity, MMP-1 expression, and collagen I synthesis. ( a ) Analysis of the inhibitory effect of Medipep-6PN on MMP-1 activity using a fluorescence assay performed in technical triplicate. The inhibition rate is expressed as a percentage compared to the untreated control group, and 1,10-phenanthroline monohydrate served as a positive control. ( b ) Evaluation of the effects of Medipep-6PN on MMP-1 mRNA expression in HDFs induced by UVB (10 mJ/cm 2 ) irradiation, performed in technical triplicate. Gray bars (non-irradiated normal control group), green bars (UVB only), blue bars (Medipep-6PN-treated group), and light blue bars (TGF-β1-treated group) are the positive control. ( c ) The results of the stimulation of collagen I production by Medipep-6PN in HDFs, performed in technical triplicate. The production rate is expressed as a percentage compared to the untreated control group, and statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test. ** p < 0.01 vs. the untreated control (CTL) group; ns, not significant. ( d ) Immunofluorescence images demonstrating the effects of Medipep-6PN on collagen I production in HDFs. First column: DAPI (blue); second column: <t>procollagen</t> <t>Iα1</t> (green); third column: merged images of nuclei and procollagen I.
Human Procollagen Iα1 Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


In vitro evaluation of the effects of Medipep-6PN on MMP-1 activity, MMP-1 expression, and collagen I synthesis. ( a ) Analysis of the inhibitory effect of Medipep-6PN on MMP-1 activity using a fluorescence assay performed in technical triplicate. The inhibition rate is expressed as a percentage compared to the untreated control group, and 1,10-phenanthroline monohydrate served as a positive control. ( b ) Evaluation of the effects of Medipep-6PN on MMP-1 mRNA expression in HDFs induced by UVB (10 mJ/cm 2 ) irradiation, performed in technical triplicate. Gray bars (non-irradiated normal control group), green bars (UVB only), blue bars (Medipep-6PN-treated group), and light blue bars (TGF-β1-treated group) are the positive control. ( c ) The results of the stimulation of collagen I production by Medipep-6PN in HDFs, performed in technical triplicate. The production rate is expressed as a percentage compared to the untreated control group, and statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test. ** p < 0.01 vs. the untreated control (CTL) group; ns, not significant. ( d ) Immunofluorescence images demonstrating the effects of Medipep-6PN on collagen I production in HDFs. First column: DAPI (blue); second column: procollagen Iα1 (green); third column: merged images of nuclei and procollagen I.

Journal: International Journal of Molecular Sciences

Article Title: In Vitro and Clinical Evaluation of the Anti-Wrinkle Efficacy of Medipep-6PN, a Novel Peptide Identified by Phage Display

doi: 10.3390/ijms27041753

Figure Lengend Snippet: In vitro evaluation of the effects of Medipep-6PN on MMP-1 activity, MMP-1 expression, and collagen I synthesis. ( a ) Analysis of the inhibitory effect of Medipep-6PN on MMP-1 activity using a fluorescence assay performed in technical triplicate. The inhibition rate is expressed as a percentage compared to the untreated control group, and 1,10-phenanthroline monohydrate served as a positive control. ( b ) Evaluation of the effects of Medipep-6PN on MMP-1 mRNA expression in HDFs induced by UVB (10 mJ/cm 2 ) irradiation, performed in technical triplicate. Gray bars (non-irradiated normal control group), green bars (UVB only), blue bars (Medipep-6PN-treated group), and light blue bars (TGF-β1-treated group) are the positive control. ( c ) The results of the stimulation of collagen I production by Medipep-6PN in HDFs, performed in technical triplicate. The production rate is expressed as a percentage compared to the untreated control group, and statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test. ** p < 0.01 vs. the untreated control (CTL) group; ns, not significant. ( d ) Immunofluorescence images demonstrating the effects of Medipep-6PN on collagen I production in HDFs. First column: DAPI (blue); second column: procollagen Iα1 (green); third column: merged images of nuclei and procollagen I.

Article Snippet: The Human procollagen Iα1 DuoSet ® ELISA kit (DY6220-05, R&D Systems, Minneapolis, MN, USA) was used, and the evaluation was performed according to the manufacturer’s provided experimental protocol.

Techniques: In Vitro, Activity Assay, Expressing, Fluorescence, Inhibition, Control, Positive Control, Irradiation, Immunofluorescence